How Much Does a Bio-One Franchise Cost?
Data from the 2025 Franchise Disclosure Document
Investment Summary
Total Investment
$135K – $221K
Franchise Fee
$60K
Royalty
the greater of the applicable Minimum Royalty ($750 to $2,250) or 7.5% of Gross Sales
Ad Fund
2.0%
Cost Breakdown
Initial Franchise Fee
The initial franchise fee for Bio-One is $60K. This one-time payment covers the right to operate under the brand, access to proprietary systems, and initial training programs.
Total Investment Range
Opening a Bio-One franchise requires a total investment of $135K – $221K. This range typically includes real estate or leasehold improvements, equipment and fixtures, initial inventory, signage, insurance, and working capital to sustain operations during the ramp-up period.
Working capital alone ranges from $15K to $25K.
Ongoing Costs
Beyond the initial investment, Bio-One franchisees pay ongoing fees. The royalty structure is: the greater of the applicable Minimum Royalty ($750 to $2,250) or 7.5% of Gross Sales. The advertising or brand fund contribution is 2.0% of gross sales. There is also a technology fee of $650.
Net Worth & Liquid Capital Requirements
Bio-One requires working capital of $15K – $25K to cover initial operating expenses. This is the liquid cash you should have available beyond the franchise fee and buildout costs.
What Can You Earn?
According to Bio-One's Item 19 financial performance representation:
This figure comes from Item 19 of the FDD. Gross sales are not the same as take-home profit. After deducting royalties, ad fund fees, rent, labor, and COGS, net income is typically a fraction of gross revenue.
How Do Banks View Bio-One?
SBA Loans Issued
74
Default Rate
24.0%
The SBA (Small Business Administration) tracks loan performance for franchise brands. Bio-One has 74 SBA-backed loans on record. The default rate is 24.0%, which is near the franchise industry average. A lower default rate generally indicates that lenders view the franchise as a safer investment, though past performance does not guarantee future results.
Next Steps
Talk to current Bio-One franchise owners
Get verified franchisee phone numbers for due diligence
See full Bio-One research
Risk analysis, unit growth, contract terms, and more
Compare Bio-One to similar franchises
Side-by-side investment, revenue, and risk comparison